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The grain is thrown Post Date: Sat, 2 Aug 2008 2:38:34 +0000
The spores are first mixed with a small proportion of the steamed grain, and this mixture then scattered over the rest. The inoculated rice is gathered in baskets and carried to the coolest part of the growing cham- ber, which normally is built underground. The grain is thrown in a heap on the floor, covered with mats and left overnight.

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In this case the preparation Post Date: Sat, 2 Aug 2008 2:22:13 +0000
After about hours it is removed and sprinkled with water. The temperature of the grain thereby falls to about Where the koji is to be used for sake" brewing this sprinkling process is omitted. In this case the preparation is termed raw koji.

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By then its appearance Post Date: Sat, 2 Aug 2008 2:10:13 +0000
In the afternoon of the second day the sprinkled grain is spread thinly on trays, placed in the growing chamber underneath other trays containing nearly finished koji. The temperature of the grain rises during the night, and by the following morning has reached a figure of about It is now gathered in small heaps on the trays and allowed to remain undisturbed for about three to four hours. By then its appearance has changed.

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The koji is left Post Date: Sat, 2 Aug 2008 1:50:37 +0000
Through the development of mycelium it has become woolly. The temperature of the heaps has reached In order to cool the grain the heaps are broken up, aerated, and again built up, to be left for another four to five hours until the temperature has risen grain is now finally scattered in thin layers on trays and the fungus allowed to spread its silky threads of mycelium throughout the mass. The koji is left on the trays for about hours until it has set to a solid cake, the ionn in which it is disposed of for industrial purposes.

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It is known under Post Date: Sat, 2 Aug 2008 1:35:11 +0000
For other types of koji, bean flour, millet or roasted barley are used, and other species of fungi, among them Asprgillua batotoe, which yields a black koji, coloured by the dark pig- mented conidiospores of the fungus (Saito). In India, according to Hutchinson and Ramayyar, an enzyme similar to koji is prepared from rice, powdered roots, and other parts of certain plants. It is known under the name of Bakhar, and is used in the manufacture of rice beers.

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In Formosa a species Post Date: Sat, 2 Aug 2008 1:18:51 +0000
It contains a great variety of fungi, the most active being Asper- gillus oryzae. Its saccharifying powers are markedly less than that of koji, and its alcohol-producing properties equally deficient owing to the inferior fermenting power of the yeast types present. In Formosa a species of Rhizopus is used for a similar pur- pose in the preparation of an alcoholic beverage, Biityn (Takeda ), and in the Malay States the Chinese inhabitants prepare a distilled beverage, using Mucor Rouxii (Bishop and Feik ) as the fungus for the hydrolysis of the raw material, rice.

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In soya koji, Saito Post Date: Sat, 2 Aug 2008 1:01:28 +0000
From the fact that various Asiatic countries have acquired what amounts to a monopoly in the industrial application of fungi to the hydrolysis of starch, it is not to be deduced that the fungi met with in Asia are exceptionally vigorous amylase producers. Nill, who undertook to ascertain whether this might be the case, found that a number of Rhizopus species could be isolated from German cereals which produced amylase to the same extent as the well-known Asiatic species, Rhizopus tonkinevisis, and AsprgiUus oryzae. In soya koji, Saito found a number of other fungi besides Aaprgillua oryzae ; among them EMzopus ja/panicus, Olado- sporium herbarum, and Penicittium glaucum.

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A description of the preparation Post Date: Sat, 2 Aug 2008 0:41:33 +0000
The active type was considered to be Aspergillus oryzae. Nishiwaki reports the preparation of, a soya koji, using Monilia sitopMla as active fungus. A description of the preparation of soya is given by Saito.

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When the fermenta- tion Post Date: Sat, 2 Aug 2008 0:25:27 +0000
The previously prepared koji, for which bean flour and wheat parts, is mixed with a steam-boiled solution of 2-8 per cent, sodium chloride in the proportions of equal parts of koji and salt- water, and the mixture stirred from time to time during the whole process of fermentation, which takes from one to one-and-a-quarter years to complete. The mash gradually acquires a deep reddish-brown colour. When the fermenta- tion is finished the mash is pressed in bags, previously soaked in the tannin containing juice of unripe date plums (Diospyros Kaki).

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In the preparation of taka-koji Post Date: Sat, 2 Aug 2008 0:08:00 +0000
On standing the filtrate separates into an oily and a watery layer. The latter, the soya, is left standing for a few days after separation from the oil and, when clear, is bottled and heated to for the purpose of sterilization. In the preparation of taka-koji, a substance which has become widely known in the form of an extract termed taka- diastase, Takamine 48 uses wheat bran instead of grain.

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