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An increased action of saccharase Post Date: Thu, 31 Jul 2008 22:51:03 +0000 Formic, phosphoric, and nitric acids, on the other hand, acted at lower concentrations on Aspergillus saccharase than on yeast saccharase. The effect of the acid on saccharase therefore was dependent both on its kations and anions. An increased action of saccharase glyoine to the reacting enzyme solution. Autor of the post: Undefined Fales and Nelson studied Post Date: Thu, 31 Jul 2008 22:33:02 +0000 Unlike maltose, sacoharase, according to Michaelis and Rona, remains soluble at all hydrogen ion concentrations. Unexplained so far is the inhibition caused by sodium chloride on the action of koji saccharase (Kellner, Moi, and Nagaoka ). Fales and Nelson studied this action in greater detail, and showed it to depend on the prevailing hydrogen ion concentration of the saccharose solution. Autor of the post: Undefined Giving a longer time for Post Date: Thu, 31 Jul 2008 22:14:00 +0000 They do not explain, however, why the action of sodium chloride should be negligible at the optimum hydrogen ion concentration of saccharase and increase with increasing deviations from this optimum. The effect of magnesia on the action of saccharase was studied by Tribot, who found that less cane sugar is hydrolysed by saccharase in a given time when magnesia is present, provided the time taken is sufficiently short. Giving a longer time for the reaction the effect apparently is the opposite, the magnesia inducing the enzyme to greater activity except at temperatures above , when the activity of the enzyme becomes independent of the presence of magnesia. Autor of the post: Undefined The action depends also Post Date: Thu, 31 Jul 2008 22:02:36 +0000 The action of silver nitrate and mercury bichloride was studied by von Euler and Svanberg. Both salts were found to have a very marked effect on the enzyme, the former in proportion to its concentration. The action depends also on the concentration of the enzyme solution and on its reaction. Autor of the post: Undefined He assumes that the proteins Post Date: Thu, 31 Jul 2008 21:48:03 +0000 It is interesting to note that the elimination of the metal from the poisoned enzyme resulted in regeneration of the enzyme. The question of the regeneration of sacoharase acquired renewed interest some time ago by the investigations of Durieux, who found that, after being heated to boiling point, the enzyme would recover as much as 10 per cent, of this behaviour of saccharase is not entirely convincing. He assumes that the proteins present in the saccharase solu- and that on precipitating they carried with them the saccha- rase held in suspension, thus removing the enzyme from the solution. Autor of the post: Undefined A reinvestigation of Durieuxs observation Post Date: Thu, 31 Jul 2008 21:30:26 +0000 A further increase of the temperature, Durieux suggests, causes the saccharase to become water soluble, aiid therefore to return into solution. Only when the temperature rises considerably above the boiling point of water is the enzyme finally destroyed. A reinvestigation of Durieuxs observation with the saccharose preparation of WiUstatter would be of great interest, since this preparation contains no protein, at least no traces of coagulable protein. Autor of the post: Undefined In this fact they see Post Date: Thu, 31 Jul 2008 21:15:06 +0000 Durieuxs actual observations on the action of heat on saccharase have been confirmed by Bertrand and Rosenblatt. These writers remark that the property of regeneration is not possessed by the maltase of yeast. In this fact they see additional support for the view that the two enzymes are different. Autor of the post: Undefined Bourquelot and Bridel find Post Date: Thu, 31 Jul 2008 21:00:46 +0000 Sacoharase, according to Bourquelot and Bridel, also has a hydrolysing action on the three carbohydrates raffinose, gentianose, and stachyose at a rate decreasing in the order named. In each case fructose is liberated, showing that saccharose constitutes a part of the molecule of each of these sugars. Bourquelot and Bridel find that the remainder of the hydrolysed carbohydrate exercises an inhibitory action not only on the inversion of these sugars but also, as already mentioned, on that of saccharose. Autor of the post: Undefined Hudson and Paine attribute Post Date: Thu, 31 Jul 2008 20:42:18 +0000 The question of the reversibility of the action of saccharase, and consequently of the biological synthesis of saccharose, is still open. Visser, who published some very interesting data on this subject, came to the conclusion that a reversibility does exist, while both Blagowestschenski and Hudson and Paine deny that this is the case. Hudson and Paine attribute the decline in rotatory power of a solution of fructose and glucose on addition of sacoharase a decline which Visser interpreted as support for his contention to the action of the hydrogen ions present on the rotation of fructose. Autor of the post: Undefined Maltose Post Date: Thu, 31 Jul 2008 20:30:15 +0000 In future investigations on this subject Bourquelot and Bridels 95 researches on the activity of saccharase in high concentra- tions of ethyl alcohol should not be overlooked. They found that the hydrolysing properties of saccharase remained practically unaffected by dissolving the enzyme in ethyl alcohol even of per cent, strength, that is, under conditions which should facilitate the elimination of any cane sugar synthesized from glucose and fructose owing to its low solubility in this strength of alcohol. Maltose. 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| 2442 | 2443 | 2444 | 2445 | 2446 | 2447 | 2448 | 2449 | 2450 | 2451 | 2452 | 2453 | 2454 | 2455 | 2456 | 2457 | 2458 | 2459 | 2460 | 2461 | 2462 | 2463 | 2464 | 2465 | 2466 | 2467 | 2468 | 2469 | 2470 | 2471 | 2472 | 2473 | 2474 | 2475 | 2476 | 2477 | 2478 | 2479 | 2480 | 2481 | 2482 | 2483 | 2484 | 2485 | 2486 | 2487 | 2488 | 2489 | 2490 | 2491 | 2492 | 2493 | 2494 | 2495 | 2496 | 2497 | 2498 | 2499 | 2500 | 2501 | 2502 | 2503 | 2504 | 2505 | 2506 | 2507 | 2508 | 2509 | 2510 | 2511 | 2512 | 2513 | 2514 | 2515 | 2516 | 2517 | 2518 | 2519 | 2520 | 2521 | 2522 | 2523 | 2524 | 2525 | 2526 | 2527 | 2528 | 2529 | 2530 | 2531 | 2532 | 2533 | 2534 | 2535 | 2536 | 2537 | 2538 | 2539 | 2540 | 2541 | 2542 | 2543 | 2544 | 2545 | 2546 | 2547 | 2548 | 2549 | 2550 | 2551 | 2552 | 2553 | 2554 | 2555 | 2556 | 2557 | 2558 | 2559 | 2560 | 2561 | 2562 | 2563 | 2564 | 2565 | 2566 | 2567 | 2568 | 2569 | 2570 | 2571 | 2572 | 2573 | 2574 | 2575 | 2576 | 2577 | 2578 | 2579 | 2580 |