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The conception of maltase Post Date: Thu, 31 Jul 2008 20:16:48 +0000
The production by micro-organisms of an enzyme capable of hydrolysing maltose was first suggested by Bourquelot 96 in 1886. As he was unable to trace the presence of glucose in the maltose-containing medium in which yeast developed, and since apparently he did not feel justified in assuming that the maltose was being assimilated directly, he concluded that a specific maltose hydrolysing enzyme would be necessary and suggested that it was being produced by the yeast cell within the cell wall. The conception of maltase as a specific enzyme was not shared by all investigators, and as late as 1894 Emil Ksoher 97 suggested that the maltose hydrolysing enzyme might be identical with saccharase.

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In addition to Rohmanns discovery Post Date: Thu, 31 Jul 2008 19:59:35 +0000
The observation made by Roh- mann 98 that commercial saccharase does not act on maltose was explained by Fischer on the assumption that the treat- ment with alcohol to which commercial saccharase is sub- jected rendered it incapable of acting on maltose. Subsequent research has proved Eischers views to be incorrect. In addition to Rohmanns discovery that maltase is destroyed by alcohol, a chemical to which saccharase is very resistant, Lintner and KrOber" have brought forward evidence to show that the optimum temperatures of the two enzymes differ materially, that of maltase being and that of saccharase The optimum hydrogen ion concentration of maltase was shown by Mohaelis and Rona 100 to be covered by the pH optimum usually attributed to saccharases.

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They also found that Post Date: Thu, 31 Jul 2008 19:47:00 +0000
At a pH value of maltase was found to be quite inactive. Michaelis and Rona point out that maltase is active only as an anion, while the uncharged molecule of saccharase is capable of hydro- lysing cane sugar. They also found that, while maltase is precipitated at its isoelectri point, saccharase remains in solution at all hydrogen ion concentrations.

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When discussing the action Post Date: Thu, 31 Jul 2008 19:36:40 +0000
A further difference between the two enzymes noted by them was that maltase is absorbed by kaolin, while sacoharase is not. Nevertheless a certain similarity between the two enzymes appears to exist. When discussing the action of aoids on sacoharase it was mentioned that this action depended as much on the anion of the acid as on its kation.

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Aubry , for instance Post Date: Thu, 31 Jul 2008 19:24:40 +0000
According to Kopaczewsky 101 this is the case also with maltase where the optimum hydrogen ion concentration, when trichloraoetic acid is used to establish it, is equal to a pH value of, but using acetic acid it amounts to a pH value of hydrolysing a-gluoosides, seeing that maltase itself is an a- glucoside. This, however, does not appear to be the case. Aubry , for instance, found that the enzyme mixture ex- tracted from Aapergittus niger, though hydrolysing maltose, had.

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Maltase is produced Post Date: Thu, 31 Jul 2008 19:13:14 +0000
no action on a-methylglucoside. Among yeasts Aubry found some types which did not produce a-methyl glucosidase though all of them produced maltase. Maltase is produced by a large number of micro-organisms other than yeast.

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He found that not only Post Date: Thu, 31 Jul 2008 18:58:17 +0000
In fact, judging from the observation of Bokorny that maltose is a highly suitable carbohydrate for a wide range of organisms, it is to be concluded that the production of maltase is common among micro-organisms. In his study of the production of maltase by Monilia sitophila Went made several interesting observations. He found that not only maltose but several other carbohydrates were capable of inducing the secretion of maltase, among them raffinose, trehalose, starch, cellulose, and xylose.

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Since Bourquelots investigations it Post Date: Thu, 31 Jul 2008 18:38:52 +0000
That this action cannot have been in the nature of a trigger action, as observed in the case of Microspira agarliguefaciens by Gray and Chalmers , is clear from the fact that maltase production in the case of Monilia sitoyihila increased pro- portionally with the increase in carbohydrate concentration until a maximum had been reached. Exceeding this point an inhibition set in which according to Went was not caused by the increase in osmotic pressure. Since Bourquelots investigations it has generally been accepted that the diffusion of maltase through the cell wall of yeast cells is impossible.

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Bourquelot and He"rissey obtained Post Date: Thu, 31 Jul 2008 18:24:15 +0000
Some years ago, however, Will- statter, Oppenheimer, and Steibelt 105 came to the conclusion that a diffusion did occur but that the maltase as it left the cell was destroyed by the acid produced during fermentation. Gentiobiose. Bourquelot and He"rissey obtained this disaccharide from gentianose and showed that the enzyme mixture of AsprgiUua niger was capable of hydrolysing it.

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This disaccharide contains two glucose Post Date: Thu, 31 Jul 2008 18:05:09 +0000
A later investigation indicated that yeast enzymes were unable to attack it. Gellobiose. This disaccharide contains two glucose resi- dues.

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