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The fact imains nevertheless Post Date: Wed, 30 Jul 2008 19:36:57 +0000
Succinio and fumaric acids mixed with gluconio acid (Wehmer ) are formed, for instance, by Aspergittusfumaricus, while the most typical citric acid producing types are capable of producing gluconio acid (Bernhauer ), and probably do so during the early stages of their action on glucose. This might appear to indicate that gluconic acid is the first intermediate oduct in the conversion of glucose to the various acidic mpoundfl dealt with, a view which has been widely held,- it which is strongly disputed by Bernhauer. The fact imains nevertheless that gluconic acid can be converted to citric acid by various fungi such as Aaprgittus fumaricus ichreyer ).

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Oxalic acid has been shown Post Date: Wed, 30 Jul 2008 19:26:29 +0000
There is another significant observation which LTOWS light on the important part played by gluoonic acid the action of fungi and glucose. Both Wehmer and jhreyer have observed that the typical fumario acid pro- icing fungus Asprgillus fumaricus loses the power of educing its characteristic acid when cultivated on artificial edia for a prolonged time and then produces chiefly gluconio id. Oxalic acid has been shown to be formed both by acetic id bacteria and by fungi not only from glucose but from [iconic acid, succinic acid, and fumario acid (Raistrick and ark ), and from citric acid (Banning ).

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In accepting this view it Post Date: Wed, 30 Jul 2008 19:12:58 +0000
It might be thought eref ore that this acid represents a further dehydrogenation oduot of the various acids mentioned, and that succinic, mario, and citric acids are intermediaries between gluconic d oxalic acid. This may be the case, but it has not yet been nfirmed experimentally. In accepting this view it should noted, however, that recent work by Kostytschev and chesnokov and by Chrzaszoz and Tinkow indicates that e formation of oxalic acid is dependent on outside influences, rticularly on the presence of a sufficient alkalinity in the Iture medium, and that it is not an essential stage in the hydrogenation of glucose by the micro-organism dealt on glucose containing media under aerobic conditions.

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Presence of alkali Post Date: Wed, 30 Jul 2008 18:54:24 +0000
In Butkewitsch and Fedoroffs experiments Mucor stolonifer yielded mainly fumaric acid COOH.COOH, but per cent, of the sugar converted could be identified as suocinio acid. Presence of alkali, or at least of calcium carbonate, and oxygen would appear to be essential for the conversion.

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Fumario acid has been shown Post Date: Wed, 30 Jul 2008 18:40:51 +0000
No work has been done to ascertain the effect of varying the concentration of the hydrogen acceptor present on the pro- portional yields of fumario and succinic acids obtained. As fumaric acid must be a dehydrogenation product of suc- cinio acid, it is to be expected that a restricted supply of hydrogen acceptors oxygen for instance must affect the conversion of glucose by Mucor stolonifer in favour of the formation of increased amounts of suocinic acid. Fumario acid has been shown to be formed both by species of Mucor [Mucor stolonifer] and of Asprgillus [Aspergillus fumaricus].

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The proposal has been advanced Post Date: Wed, 30 Jul 2008 18:30:01 +0000
Ehrlich 44 first recorded its formation by the former, Wehmer 46 by the latter. Shortage of nitrogen in the culture medium, a hydrogen ion concentration approaching neutrality, restricted supply of hydrogen acceptors (oxygen), The subject of fumario acid production by fungi is at present of theoretical interest only. The proposal has been advanced by Bailey and Potter, however, that fumaric acid might be used for the synthesis of indigo.

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In recent text-books, notably Post Date: Wed, 30 Jul 2008 18:18:05 +0000
Should this method be found industrially important the microbiological conver- sion of sugars to fumaric acid might become of practical interest. In Aapergillus the sterigmata are formed simultaneously in smaller or larger numbers over the whole surface of the vesicle, which has been formed previously at the top of the conidiophore. In recent text-books, notably in that of Thorn and Church, 51 this classification of Citromyces as a distinct genus has been abandoned, since it has been possible to show experimentally that all species of Aspergilli and some Pencillia are capable of forming citric acid, and that the characteristics of Citromyces as denned by Sartory and Bainier are insufficient to distinguish it from the Aspergilli.

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The outstanding feature of Curries Post Date: Wed, 30 Jul 2008 18:00:03 +0000
The Aspergilli giving the highest yields of citric acid over per cent, calculated on the carbohydrates decomposed were claimed by Falck and van Beyma thoe Kingma 20 to be those producing bJteckJiiQjen,_pr jawn, pigments. The moderately active jtypes, giving from 10 to 20 per cent, of acid, were those producing a yeUpjPvish-greenpigment ; the inferior types, with less than 10 per cent, yields, were those with slight pigmentation or none at all. The outstanding feature of Curries investigations is his observation that the formation of citric acid proceeds far more readily at distinctly acid reactions than at the hydrogen ion concentrations prevailing when calcium carbonate is added to the food solution.

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When this has been effected Post Date: Wed, 30 Jul 2008 17:49:17 +0000
Currie recommends an initial hydrogen ion concentration of the pH value 3-4 to 3-5. He During the first two or three days after inoculation little production of citric acid occurs as during this period the fungus spreads and covers the surface of the liquid. When this has been effected, after the third to the eighth day, the rise in acidity is very rapid, corresponding to a daily pro- duction of 2 per cent, of citric acid.

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It will be noticed Post Date: Wed, 30 Jul 2008 17:38:42 +0000
When the hydrogen ion concentration of the solution has reached a pH value of fungus development has become practically impossible, and the solution therefore is effectively protected against the growth of infecting fungi. This is impor- tant, since Wehmers early attempts failed partly owing to the mashes becoming infected by undesirable types of fungi. It will be noticed that Currie recommends the use of saccharose and not of glucose as carbohydrate.

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