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Both the percentage of moisture Post Date: Sat, 26 Jul 2008 6:10:20 +0000
It is made clear by them, however, that the action cannot be due to a specific property of the phosphoric acid radical. While the reaction of a loaf is thus of paramount impor- tance for its protection against rope, two additional causes have been elucidated which are undoubtedly of significance. Both the percentage of moisture in the bread and the tem- perature at which the loaf is stored influence the development of rope to a marked degree as already mentioned.

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The spread of the disease Post Date: Sat, 26 Jul 2008 5:53:49 +0000
Thus, it has repeatedly been found that a wet crumb, containing more than the normal 46-8 per cent, of moisture, is more susceptible to rope than a drier loaf. The influence of the temperature is perhaps less clearly defined than that of moisture, but is, nevertheless, very real. The spread of the disease within a loaf is greatly favoured by comparatively high temperatures, 39 , accord- ing to Fisher and Halton, representing the optimum.

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It was mentioned above Post Date: Sat, 26 Jul 2008 5:42:12 +0000
Rapid development is observed also at temperatures down to 25 , and only below can a loaf be stored with what Wat- kins 13 considers safety. Even here, however, protection is no more than relative, and given time there is nothing to prevent the disease from appearing in bread stored at temperatures On the whole, however, it may be claimed that rope, which occurs most frequently during the damp autumn months, can be controlled when care is taken to ensure that the hydro- gen ion concentration of the bread is kept at pH values between 5-0 and 6-2, when the loaves are cooled rapidly on removal from the oven, and when they are stored in a dry cool store-room. It was mentioned above that the first micro-organism to be associated with the appearance of rope was described by Laurent under the name of Sa panificans.

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16 Another type, described Post Date: Sat, 26 Jul 2008 5:23:05 +0000
A few years later Kratschmer and Niemilowicz investigated the case of ropi- ness in a loaf of Graham bread (wholemeal bread). They ascribed this to the activity of Sa mesentericus vulgatus, Flugg better known under Migulas name of Ba vulgatus, a type which is closely related to, but usually larger than, Ba mesentericus fuscus of Fliigge, renamed Ba mesentericus by Lehmann and Neumann. 16 Another type, described by Flugge under the name of Ba liodermos, which is closely related to Ba vulgatus, was made responsible by UfEelmann for a case of rope in rye bread.

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In the light Post Date: Sat, 26 Jul 2008 5:07:41 +0000
Vogel, who described two types of rope-producing bacteria, Ba mesentericus panis viscosi I and II, and all subsequent investigators have arrived at the conclusion that mesentericus forms are the -causative agents of rope. It has not been possible, however, to allocate the disease to one well-defined species. In the light of the conception of the mucus fermentation of carbohydrates as given in an earlier chapter this is not surprising, seeing that the property of producing rope must be possessed by all micro-organisms which can utilize bread as a food material under semi-anaerobic conditions and which under such conditions and at alkaline reactions synthesize hexoses to hexosans.

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Whether a medium such Post Date: Sat, 26 Jul 2008 4:49:31 +0000
In accepting this explanation of the appearance of mucus in rope, it must be admitted that some writers, KOmg, Spieckermann and Tillmanns, 18 for instance, attribute the appearance of mucus to the dissolution of the cell walls of the responsible micro-organisms. The evidence in favour of this view is far from convincing, and usually rests on the observa- tion that the responsible organism fails to produce mucus in a medium composed of starch and peptone. Whether a medium such as this had the required reaction on the alkalin, side of the neutral point or not is not stated.

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Of the various likely raw Post Date: Sat, 26 Jul 2008 4:38:13 +0000
Nor is it very convincingly proved that this medium is favourable for the growth of the organism in question. Since the spores of the rope-producing types of Ba mesewtericus are able to withstand the temperature of baking, and since the disease invariably manifests itself as a deteriora- tion of the crumb, it is clear that the origin of the responsible bacteria must be sought in the dough itself, or perhaps in the raw materials used for the preparation of the dough, rather than in an infection of the loaf subsequent to baking. Of the various likely raw materials, the yeast was suspected by Russell 3 in one case.

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The value of Watkinss Post Date: Sat, 26 Jul 2008 4:26:19 +0000
In the great majority of cases, how- ever, it has been possible to trace the infection to the flour itself, and Watkins 18 goes so far as to suggest that the rope- producing mesntericus forms may be regarded as members of the normal microflora of cereals. Aa a rule rye flour has been found to be infected more frequently than wheat flour, a fact which has been emphasized both by Vogel and by Fuhrmann. The value of Watkinss method is rendered doubtful by the fact that it does not guard sufficiently against the serious source of error which is introduced by omitting to adjust the hydrogen ion concentration of the crumb used as medium.

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Where that is not so Post Date: Sat, 26 Jul 2008 4:15:55 +0000
This is probably the reason why Fisher and Halton, in their experiments with Watkinss method, were sometimes unable to detect the rope bacillus in samples of flour known to con- tain it. Fisher and Halton favour the baking of a test loaf instead of the use of Watkinss method, a procedure which may meet the case where sufficient flour is available. Where that is not so, Watkinss method should be adjusted to obviate the source of error referred to.

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It was mentioned above Post Date: Sat, 26 Jul 2008 4:03:51 +0000
It would then con- stitute a valuable asset to the bacteriologist endeavouring to trace a rope infection to its source. As no method is known of eliminating an infection with rope bacteria in flour without destroying its baking properties, protection against rope can be secured only by establishing a reaction in the dough which is unfavourable for the de- velopment of the causative organism, by reducing the mois- ture content of the bread to the lowest possible figure through selection of suitable flours and yeast mixtures, and by rapid cooling and subsequent cold storage of the loaf. It was mentioned above that the aseptically removed crumb of loaves may occasionally contain lactic acid bacteria which have survived the baking process.

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